Quantitative Colorimetric Determination of Tryptophan
نویسندگان
چکیده
منابع مشابه
Quantitative colorimetric determination of tryptophan.
The methods currently used for the estimation of tryptophan are based on one of the following principles: Reaction with aldehydes in acid solution (1, Z), with glyoxylic acid (3), with mercury salts and nitrous acid (4,5), by determination after isolation from its mercury salt (6)) or by the use of Marshal’s reagent (7). Of the above mentioned methods, those based on aldehyde reactions and on t...
متن کاملQuantitative Calorimetric Determination of Tryptophan
The methods currently used for the estimation of tryptophan are based on one of the following principles: Reaction with aldehydes in acid solution (1, Z), with glyoxylic acid (3), with mercury salts and nitrous acid (4,5), by determination after isolation from its mercury salt (6)) or by the use of Marshal’s reagent (7). Of the above mentioned methods, those based on aldehyde reactions and on t...
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A simple method for the determination of tryptophan residues in proteins is described. The method involves (a) treatment of the protein with an excess of 2-hydroxy-Snitrobenzyl bromide, (b) purification of the labeled protein by gel filtration and ethanol washing, (c) measurement of the absorbance at 410 rnp to determine the number of colored groups incorporated, and (d) a determination of the ...
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All heptoses produce with orcinol in dilute HCl two characteristic colored compounds which differ greatly in their absorption spectra. Three different modifications of this reaction were found useful. Procedure i--To 2 cc. of a solution containing 20 to 100 y per cc. of ketoheptose or 5 times as much of an aldoheptose is added 0.4 cc. of concentrated HCl, sp. gr. 1.19, in a test-tube with a gro...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1960
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)69467-0